3V6C
Crystal Structure of USP2 in complex with mutated ubiquitin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-05 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.735, 84.077, 86.915 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 84.080 - 1.700 |
R-factor | 0.17844 |
Rwork | 0.176 |
R-free | 0.22018 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2hd5 |
RMSD bond length | 0.020 |
RMSD bond angle | 1.983 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 84.080 | 1.750 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.058 | |
Number of reflections | 45718 | |
<I/σ(I)> | 19.49 | 2.31 |
Completeness [%] | 97.4 | 72.3 |
Redundancy | 6.39 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 21% PEG 3350,0.2 M CaCl, 0.1 Bis-Tris pH 7.0, glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293K |