3V5W
Human G Protein-Coupled Receptor Kinase 2 in Complex with Soluble Gbetagamma Subunits and Paroxetine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2011-03-21 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 194.973, 71.011, 111.169 |
| Unit cell angles | 90.00, 110.41, 90.00 |
Refinement procedure
| Resolution | 24.530 - 2.070 |
| R-factor | 0.19614 |
| Rwork | 0.193 |
| R-free | 0.24668 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.201 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.110 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 55040 | |
| <I/σ(I)> | 16.8 | 1.5 |
| Completeness [%] | 61.8 | 30 |
| Redundancy | 2.6 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 100 mM MES pH 5.8, 200 mM NaCl, and 8% (w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
