3UWP
Crystal structure of Dot1l in complex with 5-iodotubercidin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-23 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.91963 |
Spacegroup name | P 65 |
Unit cell lengths | 149.784, 149.784, 53.521 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.050 |
R-factor | 0.196 |
Rwork | 0.195 |
R-free | 0.22310 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.327 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.120 |
High resolution limit [Å] | 2.050 | 4.420 | 2.050 |
Rmerge | 0.098 | 0.046 | 0.973 |
Number of reflections | 43269 | ||
<I/σ(I)> | 8.8 | ||
Completeness [%] | 99.6 | 99.5 | 96.7 |
Redundancy | 5.9 | 6.1 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.6 | 291 | 3.5M sodium formate, 0.1M sodium acetate, pH 4.6, vapor diffusion, temperature 291K |