3UVO
Crystal structure of WDR5 in complex with the WDR5-interacting motif of SET1B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-06-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.12718 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.270, 69.920, 88.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.010 - 2.200 |
| R-factor | 0.1884 |
| Rwork | 0.187 |
| R-free | 0.21870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h13 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.350 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 15927 | |
| <I/σ(I)> | 43.7 | 6.9 |
| Completeness [%] | 98.7 | 86.8 |
| Redundancy | 7 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 0.1 M ammonium sulfate, 25% PEG3350, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
