3UVN
Crystal structure of WDR5 in complex with the WDR5-interacting motif of SET1A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2011-06-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.12718 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.890, 47.100, 102.520 |
| Unit cell angles | 90.00, 108.21, 90.00 |
Refinement procedure
| Resolution | 42.401 - 1.792 |
| R-factor | 0.1953 |
| Rwork | 0.194 |
| R-free | 0.23030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h13 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.401 | 2.200 |
| High resolution limit [Å] | 1.792 | 1.792 |
| Number of reflections | 68329 | |
| <I/σ(I)> | 22 | 4.8 |
| Completeness [%] | 98.1 | 91.1 |
| Redundancy | 7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 25% PEG3350, 0.1 M sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
