3UVI
tRNA-guanine transglycosylase C158S C281S W326E E339Q mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.91841 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.019, 64.984, 70.488 |
Unit cell angles | 90.00, 95.60, 90.00 |
Refinement procedure
Resolution | 35.076 - 1.550 |
R-factor | 0.166 |
Rwork | 0.165 |
R-free | 0.19070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pud |
RMSD bond length | 0.006 |
RMSD bond angle | 1.054 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 58805 | |
<I/σ(I)> | 16.08 | 2.78 |
Completeness [%] | 99.0 | 89.7 |
Redundancy | 3 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 291 | Sodium iodide 0.2M, PEG 3350 20% w/v, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 291K |