3US2
Structure of p63 DNA Binding Domain in Complex with a 19 Base Pair A/T Rich Response Element Containing Two Half Sites with a Single Base Pair Overlap
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320357 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.739, 180.544, 98.174 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 59.869 - 4.200 |
| R-factor | 0.3402 |
| Rwork | 0.326 |
| R-free | 0.33420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.747 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.788 | 4.300 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.112 | 0.453 |
| Number of reflections | 14781 | |
| <I/σ(I)> | 5.8 | 1.6 |
| Completeness [%] | 96.8 | 94.6 |
| Redundancy | 2.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 0.2 M ammonium formate, 0.1 M Bis-Tris, pH 6.8, 12% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
