3UNB
Mouse constitutive 20S proteasome in complex with PR-957
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-08 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 171.720, 198.590, 226.750 |
| Unit cell angles | 90.00, 106.59, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.900 |
| R-factor | 0.23178 |
| Rwork | 0.230 |
| R-free | 0.27259 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iru |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.885 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 | 0.481 |
| Number of reflections | 308934 | |
| <I/σ(I)> | 9.4 | 2 |
| Completeness [%] | 96.0 | 96.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.2 M Sodium formate, 40% MPD , pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
