3UK6
Crystal Structure of the Tip48 (Tip49b) hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9720 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 113.070, 186.280, 129.860 |
| Unit cell angles | 90.00, 108.95, 90.00 |
Refinement procedure
| Resolution | 43.190 - 2.950 |
| R-factor | 0.2425 |
| Rwork | 0.241 |
| R-free | 0.26420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.190 | 3.030 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.054 | 0.690 |
| Number of reflections | 103557 | |
| <I/σ(I)> | 16.7 | 1.91 |
| Completeness [%] | 97.0 | 99.2 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Tris-HCL, pH 8.0, 20% PEG 3000, 5% glucose, galactose or trehalose, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
