3UJ0
Crystal structure of the inositol 1,4,5-trisphosphate receptor with ligand bound form.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.166, 78.720, 134.107 |
| Unit cell angles | 90.00, 124.49, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.26 |
| Rwork | 0.258 |
| R-free | 0.30780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.116 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.600 |
| Number of reflections | 71417 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 293 | Na Citrate, PEG-6000, pH 8.4, vapor diffusion, hanging drop, temperature 293K |
