3UEE
Crystal structure of human Survivin K62A mutant bound to N-terminal histone H3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.888, 70.872, 82.037 |
| Unit cell angles | 90.00, 129.52, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.610 |
| R-factor | 0.1875 |
| Rwork | 0.185 |
| R-free | 0.23679 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3uec |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.790 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.055 | 0.430 |
| Number of reflections | 15257 | |
| <I/σ(I)> | 38.3 | 2.8 |
| Completeness [%] | 99.1 | 93.6 |
| Redundancy | 4.1 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 12% PEG 3350, 0.12 M succinic acid, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
