3UCR
Crystal structure of the immunoreceptor TIGIT IgV domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 99.964, 99.964, 117.519 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.045 - 2.627 |
| R-factor | 0.2184 |
| Rwork | 0.215 |
| R-free | 0.27400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.183 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.045 | 2.690 |
| High resolution limit [Å] | 2.627 | 2.627 |
| Rmerge | 0.600 | |
| Number of reflections | 20690 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 99.9 | 67 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 1.5 M (NH4)2SO4 and 0.1M HEPES pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
