3UA8
Crystal Structure Analysis of a 6-Amino Quinazolinedione Sulfonamide bound to human GluR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.800 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 51.181, 104.548, 49.441 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.1854 |
| Rwork | 0.184 |
| R-free | 0.21540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r7x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.181 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.082 | 1.900 |
| Rmerge | 0.062 | 0.042 | 0.141 |
| Number of reflections | 21361 | ||
| <I/σ(I)> | 16.8 | ||
| Completeness [%] | 98.8 | 89.8 | 99.5 |
| Redundancy | 10.9 | 9.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | HANGING DROP | 7.5 | 277 | 20% w/v 2-propanol, 10% w/v PEG4000, 100 mM HEPES, pH 7.5, HANGING DROP, temperature 277K |
