3U8D

Functionally selective inhibition of Group IIA phospholipase A2 reveals a role for vimentin in regulating arachidonic acid metabolism

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	77
Detector technology	CCD
Collection date	2006-08-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.90020
Spacegroup name	P 31 2 1
Unit cell lengths	74.422, 74.422, 93.674
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.820 - 1.805
R-factor	0.1264
Rwork	0.121
R-free	0.18930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.209
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall
Low resolution limit [Å]	37.178
High resolution limit [Å]	1.801
Number of reflections	28140
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	0.1 M Tris-HCl pH 7.4, 10 mM CaCl2, 0.5 mM beta-octyl glucoside and 4 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K