3U53
Crystal structure of human Ap4A hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 |
| Unit cell lengths | 72.485, 72.485, 133.491 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.705 |
| R-factor | 0.21654 |
| Rwork | 0.213 |
| R-free | 0.28427 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kt9 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.609 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.775 |
| High resolution limit [Å] | 2.705 | 2.705 |
| Rmerge | 0.098 | 0.376 |
| Number of reflections | 17881 | |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 100 | |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 0.1M Sodium citrate tribasic dihydrate, 1.2M Lithium sulfate, 0.2M Ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
