3TZ7
Kinase domain of cSrc in complex with RL103
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.541700 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.550, 64.280, 75.400 |
| Unit cell angles | 79.28, 87.38, 90.20 |
Refinement procedure
| Resolution | 28.580 - 3.300 |
| R-factor | 0.193 |
| Rwork | 0.189 |
| R-free | 0.25630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f3v |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.224 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (1.3.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 16.000 | 3.300 |
| Rmerge | 0.063 | 0.020 | 0.171 |
| Rmeas | 0.027 | 0.234 | |
| Number of reflections | 11298 | 78 | 954 |
| <I/σ(I)> | 15.24 | 38.45 | 5.64 |
| Completeness [%] | 95.8 | 75 | 95.6 |
| Redundancy | 2.21 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10% PEG 20000, 15% glycerol, 100mM MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
