3TM4
Crystal structure of Trm14 from Pyrococcus furiosus in complex with S-adenosylmethionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00150 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.080, 45.170, 121.430 |
| Unit cell angles | 90.00, 91.79, 90.00 |
Refinement procedure
| Resolution | 49.530 - 1.950 |
| R-factor | 0.18258 |
| Rwork | 0.182 |
| R-free | 0.19592 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tlj |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.400 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.073 | 0.544 |
| Number of reflections | 65283 | |
| <I/σ(I)> | 18.29 | 3.6 |
| Completeness [%] | 99.5 | 98.9 |
| Redundancy | 7.1 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 100 mM Tris-acetate, 32% PEG 4000, 15% glycerol, crystal was soaked in crystallization solution containing 1 mM S-adenosylmethionine, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
