3TGX

IL-21:IL21R complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	77
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	82.230, 151.120, 364.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	80.220 - 2.800
R-factor	0.244
Rwork	0.242
R-free	0.27300
Structure solution method	SIRAS
RMSD bond length	0.025
RMSD bond angle	1.790
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.700	3.000
High resolution limit [Å]	2.800	2.800
Rmerge	0.187	0.483
<I/σ(I)>	30.1	3.6
Completeness [%]	99.7	69.1
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291.3	1,8-1,9 M di-Ammonium sulphate and 0.1 M Sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 291.3K