3TG5
Structure of SMYD2 in complex with p53 and SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-05-10 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.360, 71.670, 121.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.750 - 2.300 |
| R-factor | 0.24215 |
| Rwork | 0.241 |
| R-free | 0.26317 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tg4 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.988 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.800 | 2.320 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 20928 | |
| Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1M Hepes (pH 7.5), 25% PEG 3350, VAPOR DIFFUSION, temperature 293K |
