3T7A
Crystal structure of the catalytic domain of human diphosphoinositol pentakisphosphate kinase 2 (PPIP5K2) in complex with ADP at pH 5.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-02-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.973, 110.807, 41.381 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.280 - 1.700 |
R-factor | 0.18095 |
Rwork | 0.179 |
R-free | 0.20635 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3t54 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.189 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 45377 | |
<I/σ(I)> | 28.5 | 3 |
Completeness [%] | 99.2 | 94.8 |
Redundancy | 5.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 12% (w/v) PEG 3350, 20 mM MgCl2, 0.1 M HEPES, 1 mM ATP, 2 mM CdCl2 Soaking under pH5.2 overnight , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |