3SZM
STRUCTURE OF HUMAN MICROCEPHALIN (MCPH1) TANDEM BRCT DOMAINS IN COMPLEX WITH A GAMMA-H2AX PHOSPHOPEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-15 |
| Wavelength(s) | 1.0079 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.810, 82.230, 104.420 |
| Unit cell angles | 72.10, 89.87, 80.94 |
Refinement procedure
| Resolution | 31.370 - 2.630 |
| R-factor | 0.202 |
| Rwork | 0.201 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.046 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | DM |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.064 | 0.471 |
| Number of reflections | 59208 | |
| <I/σ(I)> | 13.57 | 2 |
| Completeness [%] | 90.2 | 97.8 |
| Redundancy | 2.6 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 20% PEG 4000, 0.1M (NH4)H2PO4, 0.1M BIS-TRIS-PROPANE , PH 4.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 295K |
