3SM4
Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with a 5'-Phosphorylated 14-mer/12-mer Duplex and Magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-03 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 65 |
| Unit cell lengths | 78.391, 78.391, 247.534 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.890 - 1.880 |
| R-factor | 0.16113 |
| Rwork | 0.160 |
| R-free | 0.18578 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1avq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.919 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.900 | 1.980 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.084 | 0.480 |
| Number of reflections | 65978 | |
| <I/σ(I)> | 20.3 | 4.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 19% PEG3350, 0.2 M sodium bromide, 5 mM magnesium chloride, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
