3SM4
Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with a 5'-Phosphorylated 14-mer/12-mer Duplex and Magnesium
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Exonuclease | polymer | 229 | 26164.8 | 3 | UniProt (P03697) Pfam (PF09588) | Enterobacteria phage lambda | |
| 2 | D (D) | 5'-D(*TP*CP*GP*GP*TP*AP*CP*AP*GP*TP*AP*G)-3' | polymer | 12 | 3702.4 | 1 | |||
| 3 | E (E) | 5'-D(P*AP*GP*CP*TP*AP*CP*TP*GP*TP*AP*CP*CP*GP*A)-3' | polymer | 14 | 4264.8 | 1 | |||
| 4 | F, J (A, C) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
| 5 | G, H (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 6 | I, K (B, E) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 7 | L, M, N, O, P (A, B, C, D, E) | water | water | 18.0 | 584 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 226 (UniProt: P03697)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 131 | Lys 131 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 86461.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 309.5 | |
| All* | Total formula weight | 86771.0 |
