3SM4

Crystal Structure of the K131A Mutant of Lambda Exonuclease in Complex with a 5'-Phosphorylated 14-mer/12-mer Duplex and Magnesium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C	Exonuclease	polymer	229	26164.8	3	UniProt (P03697) Pfam (PF09588) In PDB	Enterobacteria phage lambda
2	D	5'-D(*TP*CP*GP*GP*TP*AP*CP*AP*GP*TP*AP*G)-3'	polymer	12	3702.4	1
3	E	5'-D(P*AP*GP*CP*TP*AP*CP*TP*GP*TP*AP*CP*CP*GP*A)-3'	polymer	14	4264.8	1
4	A, C	PHOSPHATE ION	non-polymer		95.0	2	Chemie (PO4)
5	A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
6	B, E	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7		water	water		18.0	584	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 226 (UniProt: P03697)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 131	Lys 131	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	86461.5
Non-Polymers*	Number of molecules	6
	Total formula weight	309.5
All*	Total formula weight	86771.0

*Water molecules are not included.