3SL9
X-ray structure of Beta catenin in complex with Bcl9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-20 |
| Detector | ADSC QUANTUM 4r |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 160.890, 80.700, 87.940 |
| Unit cell angles | 90.00, 102.00, 90.00 |
Refinement procedure
| Resolution | 29.430 - 2.200 |
| R-factor | 0.197 |
| Rwork | 0.194 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gl7 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.969 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.070 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.050 | 0.050 |
| Number of reflections | 54333 | |
| <I/σ(I)> | 17.1 | 17.1 |
| Completeness [%] | 97.2 | 97.2 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 298 | 0.12M ethylene glycol, 1M MES 62% MES, 1M Imidazol 38%, 10% PEG 4K, 20% glycerol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
