3SKU
Herpes simplex virus glycoprotein D bound to the human receptor nectin-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-14 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 188.003, 188.003, 185.004 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 4.000 |
| R-factor | 0.267 |
| Rwork | 0.266 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c36 1NEU AND 3ALP |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.747 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.100 | 4.100 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Number of reflections | 31947 | |
| <I/σ(I)> | 8 | 1.7 |
| Completeness [%] | 99.9 | 99.2 |
| Redundancy | 2.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.2 | 293 | 1.0 M Na2HPO4/KH2PO4 pH 7.2 and 300 mM NH4SO4, VAPOR DIFFUSION, temperature 293K |
