3SHT
Crystal structure of human MCPH1 tandem BRCT domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.469, 113.665, 74.582 |
| Unit cell angles | 90.00, 91.04, 90.00 |
Refinement procedure
| Resolution | 32.580 - 1.950 |
| R-factor | 0.2041 |
| Rwork | 0.202 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ing |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.401 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
| Rmerge | 0.045 | 0.024 | 0.212 |
| Number of reflections | 54633 | ||
| <I/σ(I)> | 13.4 | ||
| Completeness [%] | 97.1 | 97.3 | 87.5 |
| Redundancy | 3.4 | 3.4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 281 | 100mM HEPES, 10% PEG6000, 5% MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
