3SHJ
Proteasome in complex with hydroxyurea derivative HU10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-04-27 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.117, 300.565, 144.058 |
| Unit cell angles | 90.00, 112.76, 90.00 |
Refinement procedure
| Resolution | 49.765 - 2.800 |
| R-factor | 0.238 |
| Rwork | 0.238 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ryp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.765 | 2.800 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.089 | 0.508 |
| Number of reflections | 250186 | |
| <I/σ(I)> | 9.4 | 2 |
| Completeness [%] | 91.7 | 90.2 |
| Redundancy | 2.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20 mM magnesium acetate, 11% PEG, 100 mM MES/NaOH, pH 7.0, crystals grow to their final size within a week, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
