3SHF
Crystal structure of the R265S mutant of full-length murine Apaf-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.880, 111.820, 244.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.550 |
| Rwork | 0.230 |
| R-free | 0.30460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sfz |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.800 | |
| High resolution limit [Å] | 3.550 | 20.000 | 3.550 |
| Rmerge | 0.081 | 0.020 | 0.443 |
| Number of reflections | 24831 | 155 | 4499 |
| <I/σ(I)> | 21.7 | 67.94 | 4.44 |
| Completeness [%] | 99.5 | 87.1 | 99.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 100 mM HEPES pH 7.0, 1.4 M sodium malonate, 4% v/v gamma-butyrolactone, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
