3SFX
Cryptococcus neoformans protein farnesyltransferase in complex with FPT-II and tipifarnib
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-05-23 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.11 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 142.176, 142.176, 130.096 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.470 - 2.000 |
R-factor | 0.18107 |
Rwork | 0.180 |
R-free | 0.19905 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.264 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Number of reflections | 85314 |
Completeness [%] | 100.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 290 | PEG4K, CAPSO pH 9.5, Lithium sulfate, sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 290K |