3SE3

human IFNa2-IFNAR ternary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-03
Detector	ADSC QUANTUM 315r
Spacegroup name	P 61 2 2
Unit cell lengths	93.370, 93.370, 401.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.780 - 4.000
Rwork	0.251
R-free	0.31100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3se4 3s9d
RMSD bond length	0.010
RMSD bond angle	1.817
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	4.100
High resolution limit [Å]	4.000	4.000
Number of reflections	9537
<I/σ(I)>	11.7	2.6
Completeness [%]	99.8	100
Redundancy	11	11.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	293	15% (w/v) PEG 3350, 100 mM Na malonate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K