3SDL
Crystal structure of human ISG15 in complex with NS1 N-terminal region from influenza B virus, Northeast Structural Genomics Consortium Target IDs HX6481, HR2873, and OR2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.009, 56.142, 74.020 |
| Unit cell angles | 70.59, 84.24, 83.91 |
Refinement procedure
| Resolution | 36.715 - 2.290 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z2m 1xeq |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.648 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.062 | 0.369 |
| Number of reflections | 24402 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 93.3 | 96.4 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 293 | 15% PEG3350, 1% dioxane, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, EVAPORATION |
