3SBC
Crystal structure of Saccharomyces cerevisiae TSA1C47S mutant protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-08-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.43 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 239.983, 51.961, 192.355 |
| Unit cell angles | 90.00, 92.33, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.20517 |
| Rwork | 0.202 |
| R-free | 0.27350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.783 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.680 | 2.798 |
| Number of reflections | 55115 | |
| Completeness [%] | 94.8 | 97.7 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1 M sodium citrate, 10% PEG 3000, 0.1 M sodium fluoride, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
