3RY6
Complex of fcgammariia (CD32) and the FC of human IGG1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 153.380, 255.550, 58.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.710 - 3.800 |
| R-factor | 0.252 |
| Rwork | 0.252 |
| R-free | 0.42600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e4k |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Number of reflections | 11766 | |
| <I/σ(I)> | 10.8 | 3.78 |
| Completeness [%] | 98.7 | 99.7 |
| Redundancy | 5 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 20% (W/V) PEG 3350, 5MM TRIS, 0.15M NACL, 2MM MOPS, 0.2M TRI-POTASSIUM ACETATE, PH 7.40, VAPOR DIFFUSION, TEMPERATURE 291.0K |
