3ROV
Insulin's biosynthesis and activity have opposing structural requirements: a new factor in neonatal diabetes mellitus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-09 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.662, 61.772, 46.038 |
| Unit cell angles | 90.00, 105.50, 90.00 |
Refinement procedure
| Resolution | 44.360 - 2.300 |
| Rwork | 0.218 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1znj |
| RMSD bond angle | 0.008 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.360 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.057 | 0.232 |
| Number of reflections | 10607 | |
| <I/σ(I)> | 12.1 | 32.8 |
| Completeness [%] | 95.9 | 91.1 |
| Redundancy | 2.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 298 | 0.05 M sodium citrate, 1% phenol, 0.04% zinc acetate, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
