3RN8

Crystal Structure of iGluR2 Ligand Binding Domain and Symmetrical Carboxyl Containing Potentiator

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-08
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	113.889, 163.232, 47.356
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.960 - 1.700
R-factor	0.19092
Rwork	0.189
R-free	0.22418
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.614
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	X-PLOR
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.055
Number of reflections	96319
<I/σ(I)>	25.8
Completeness [%]	98.5	95.1
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	PEG8000, Sodium Cacodylate, Zinc Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K