3RMC
Crystal Structure of a replicative DNA polymerase bound to DNA containing Thymine Glycol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 133.991, 123.767, 163.637 |
| Unit cell angles | 90.00, 95.57, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| Rwork | 0.222 |
| R-free | 0.28200 |
| Starting model (for MR) | 2dy4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.307 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.085 | 0.034 | 0.695 |
| Number of reflections | 107001 | ||
| <I/σ(I)> | 12.1 | ||
| Completeness [%] | 99.9 | 99.4 | 99.9 |
| Redundancy | 4.2 | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 297 | 4.5 % PEG 20,000, 150 mM Na acetate, 125 mM Mg acetate, 100 mM TrisHCl, 1% glycerol, 10mM Phenol and 2 mM beta-mercaptoethanol, vapor diffusion, hanging drop, temperature 297K, pH 6.8, VAPOR DIFFUSION, HANGING DROP |
