3RL0
Truncated SNARE complex with complexin (P1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2010-04-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.745, 127.357, 142.725 |
| Unit cell angles | 107.49, 90.01, 90.05 |
Refinement procedure
| Resolution | 30.000 - 3.800 |
| R-factor | 0.308 |
| Rwork | 0.306 |
| R-free | 0.34500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.035 |
| RMSD bond angle | 1.011 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Completeness [%] | 83.5 | 80.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 294 | 13-15% polyethyleneglycol (PEG) 5000MME, 0.2 M ammonium sulfate, 0.01 M EDTA, and 0.1 M Tris pH 7.5, VAPOR DIFFUSION, temperature 294K |
