3REA
HIV-1 Nef protein in complex with engineered Hck-SH3 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-29 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.692, 65.692, 279.068 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.16989 |
| Rwork | 0.168 |
| R-free | 0.20544 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1efn |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.823 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | REFMAC (5.5.0102) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.770 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 40486 | |
| <I/σ(I)> | 18.2 | |
| Completeness [%] | 99.5 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | 100 mM Tris buffer, 5% ethylne glycol, 10% PEG 8000, 0.2 M MgCl2, 15 mM MnCl2, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
