3QWQ
Crystal structure of the extracellular domain of the epidermal growth factor receptor in complex with an adnectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-05 |
Detector | RAYONIX MX-300 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.000, 72.100, 262.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.470 - 2.750 |
R-factor | 0.204 |
Rwork | 0.202 |
R-free | 0.24600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EGFR FROM PDB ENTRY 1NQL AND ADNECTIN FROM PDB ENTRY 1FNF RESIDUES 1421-1437 1444-1466 1470-1491 1502-1509 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 ((DENZO)) |
Data scaling software | HKL-2000 ((SCALEPACK)) |
Phasing software | PHASER |
Refinement software | BUSTER-TNT (BUSTER 2.13.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.069 | 0.552 |
Number of reflections | 34285 | |
<I/σ(I)> | 23.1 | 3.1 |
Completeness [%] | 99.6 | 100 |
Redundancy | 4.8 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 293 | 60% Tacsimate, pH 7.0, vapor diffusion, hanging drop, temperature 293K |