3QPB
Crystal Structure of Streptococcus Pyogenes Uridine Phosphorylase Reveals a Subclass of the NP-I Superfamily
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 1 |
| Unit cell lengths | 92.021, 91.578, 170.180 |
| Unit cell angles | 78.46, 82.33, 60.12 |
Refinement procedure
| Resolution | 50.000 - 1.820 |
| Rwork | 0.177 |
| R-free | 0.20300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.820 |
| Number of reflections | 404352 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 18% PEG 4000, 16% isopropanol, and 0.1 M sodium citrate pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
