3QEG
Crystal structure of human N12-i2 Fab, an ADCC and neutralizing anti-HIV-1 Env antibody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979464 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 78.906, 78.906, 315.147 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.20483 |
| Rwork | 0.203 |
| R-free | 0.23612 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rz8 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.794 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.335 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.094 | 0.795 |
| Number of reflections | 43613 | |
| <I/σ(I)> | 40 | 2.5 |
| Completeness [%] | 99.0 | 99.7 |
| Redundancy | 20.2 | 15.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 294 | 0.01 M Ni (II) chloride hexahydrate, 0.1 M Tris HCl, pH 8.5, 20% Polyethylene glycol monomethyl ether 2,000, , VAPOR DIFFUSION, HANGING DROP, temperature 294K |
