3QAZ
IL-2 mutant D10 ternary complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2010-09-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 118.226, 118.099, 236.115 |
| Unit cell angles | 99.86, 99.86, 99.74 |
Refinement procedure
| Resolution | 52.191 - 3.802 |
| R-factor | 0.292 |
| Rwork | 0.291 |
| R-free | 0.34400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.085 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_621)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.200 | 3.938 |
| High resolution limit [Å] | 3.800 | 3.802 |
| Rmerge | 0.428 | |
| Number of reflections | 111113 | |
| <I/σ(I)> | 2.08 | |
| Completeness [%] | 93.4 | 98.7 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 100 mM Bis-Tris, 200 mM NH4SO4, 25% PEG-3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
