3PUA
PHF2 Jumonji-NOG-Ni(II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 109.575, 65.767, 66.612 |
| Unit cell angles | 90.00, 102.09, 90.00 |
Refinement procedure
| Resolution | 32.883 - 1.890 |
| R-factor | 0.1667 |
| Rwork | 0.164 |
| R-free | 0.21020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kv4 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.982 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.890 | 1.960 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.087 | 0.563 |
| Number of reflections | 34150 | |
| <I/σ(I)> | 14.2 | 2.2 |
| Completeness [%] | 91.5 | 78.9 |
| Redundancy | 5.1 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 18-30% polyethylene glycol 3350 and 100 mM NaCitrate, pH 5.2-6.0, VAPOR DIFFUSION |
