3PEG
Crystal structure of Neurofibromins Sec14-PH module containing a patient derived duplication (TD)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-15 |
| Detector | ADSC |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 104.600, 104.600, 116.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.570 - 2.524 |
| R-factor | 0.2254 |
| Rwork | 0.223 |
| R-free | 0.26430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.613 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.570 | 2.670 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Number of reflections | 12507 | |
| Completeness [%] | 94.5 | 75.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289.15 | 0.05M HEPES, 39% PEG 400, 0.2M (NH4)2SO4, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289.15K |
