3P8B

X-ray crystal structure of Pyrococcus furiosus transcription elongation factor Spt4/5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Detector technology	CCD
Collection date	2009-11-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9181
Spacegroup name	P 2 21 21
Unit cell lengths	40.532, 87.213, 133.107
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.23928
Rwork	0.236
R-free	0.29234
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.024
RMSD bond angle	1.991
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.800
Number of reflections	44007

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	295	0.1 M Bis-Tris (pH 5.5), 0.2 M NaCl, and 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 295K