3P6Z
Structural basis of thrombin mediated factor V activation: essential role of the hirudin-like sequence Glu666-Glu672 for processing at the heavy chain-B domain junction
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2009-03-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9725 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.208, 62.259, 67.510 |
| Unit cell angles | 99.38, 110.46, 92.26 |
Refinement procedure
| Resolution | 31.920 - 1.700 |
| R-factor | 0.20059 |
| Rwork | 0.200 |
| R-free | 0.23876 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ppb |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.032 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.140 | 1.790 |
| High resolution limit [Å] | 1.690 | 1.696 |
| Rmerge | 0.080 | |
| Number of reflections | 81788 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 94.2 | 89.6 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM MOPS/HEPES-Na, pH 7.5, 12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (v/v) 2-methylpentane-2,4-diol (MPD), 30 mM Ca2+, 30 mM Mg2+, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
