3P35
Polo-like kinase I Polo-box domain in complex with MQSpSPL phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Collection date | 2010-04-22 |
| Wavelength(s) | 0.9334 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.893, 95.828, 65.964 |
| Unit cell angles | 90.00, 116.34, 90.00 |
Refinement procedure
| Resolution | 59.130 - 2.090 |
| R-factor | 0.20922 |
| Rwork | 0.206 |
| R-free | 0.27315 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1UMW (chain A) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.020 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 59.130 |
| High resolution limit [Å] | 2.090 |
| Number of reflections | 38299 |
| Completeness [%] | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M potassium formate, 20% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
