3OZJ

Crystal structure of human retinoic X receptor alpha complexed with bigelovin and coactivator SRC-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	277
Detector technology	CCD
Collection date	2010-08-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9753
Spacegroup name	P 21 21 21
Unit cell lengths	65.400, 66.590, 111.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.290 - 2.100
R-factor	0.24467
Rwork	0.245
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2p1t
RMSD bond length	0.012
RMSD bond angle	1.723
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.290
High resolution limit [Å]	2.100
Number of reflections	25813
Completeness [%]	88.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	8% v/v Tacsimate pH7.5, 20% w/v PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K