3OY9

Crystal structure of the Prototype Foamy Virus (PFV) intasome in complex with manganese at 2.55 resolution

Replaces:  3L2S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	AREA DETECTOR
Collection date	2010-04-26
Detector	ADSC
Wavelength(s)	0.97950
Spacegroup name	P 41 21 2
Unit cell lengths	160.090, 160.090, 123.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.210 - 2.550
R-factor	0.1986
Rwork	0.197
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3l2r
RMSD bond length	0.011
RMSD bond angle	1.496
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.206	39.206	2.690
High resolution limit [Å]	2.550	8.060	2.550
Rmerge		0.034	0.931
Total number of observations		8693	40335
Number of reflections	52142
<I/σ(I)>	14.1	54.7	2
Completeness [%]	98.9	91.6	99
Redundancy	5.5	5.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	1.35 M ammonium sulfate, 25% (v/v) glycerol, 4.8% (v/v) 1,6-hexanediol, 50 mM Mes-NaOH, 1mM EDTA, pH 6.5, vapor diffusion, hanging drop, temperature 291K