3OLA
Poliovirus polymerase elongation complex with 2'-deoxy-CTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-12 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.562, 60.560, 192.832 |
| Unit cell angles | 83.57, 83.56, 77.59 |
Refinement procedure
| Resolution | 46.709 - 2.550 |
| R-factor | 0.2288 |
| Rwork | 0.226 |
| R-free | 0.28350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ra6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.216 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.710 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.086 | 0.583 |
| Number of reflections | 106397 | |
| <I/σ(I)> | 4.3 | 1.2 |
| Completeness [%] | 96.4 | 93.6 |
| Redundancy | 1.96 | 1.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 283 | 95 mM trisodium citrate, pH 5.6-5.9, 6.5-7.0% (v/v) isopropanol, 16.5-18.0% (w/v) PEG4000, 5% (v/v) glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 283K |
